Alexandru I. Petrisor, PhD Candidate
Alan W. Decho, PhD
Laboratory of Microbial Interactions
Department of Environmental Health Sciences
Arnold School of Public Health
University of South Carolina
In an attempt to develop a methodology of analyzing and classifying images obtained using confocal scanning laser microscopy, similar to analyzing satellite images, to answer specific research questions relevant to microbiology, the reconstruction and computation, or at least estimation of biovolumes played an important role. Even though the final goal is to use this approach for the analysis of microbial images, for the most part of this particular research project, polymeric microspheres of known sizes were used to create a ground truth. This paper is presenting particular aspects related to the process, identifying potential difficulties and presenting several possible approaches to overcome these difficulties.